Osteoarthritis (OA) is a common chronic degenerative joint disease in clinical practice with a high prevalence, especially in the elderly. Traditional Chinese Medicine (TCM) believes that OA belongs to the category of "Bi syndrome" and the "bone Bi syndrome". The etiology and pathogenesis lie in the deficiency of the liver and kidney, the deficiency of Qi and blood, and external exposure to wind, cold, and dampness. Epimedium is a yang-reinforcing herb in TCM, which can tonify the liver and kidney, strengthen muscles and bones, dispel wind, cold and dampness, and can treat both the symptoms and the root cause of "bone Bi syndrome". In addition, Epimedium contains a large number of ingredients. Through modern science and technology, more than 270 compounds have been found in Epimedium, among which flavonoids are the main active ingredients. Therefore, our study will review the effects and mechanisms of genus Epimedium in treating OA from two aspects: (1) Introduction of Epimedium and its main active ingredients; (2) Effects of Epimedium and its active ingredients in treating OA and relevant signaling pathways, in order to provide more ideas for OA treatment.

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Monotropein, a principal natural compound in iridoid glycosides extracted from Morindae officinalis radix, has potent pharmacological activities. To understand and utilize monotropein, we systematically summarized the studies on monotropein, including its biosynthetic pathway, physicochemical properties, pharmacokinetics, and pharmacology. Interestingly, we found that the multiple bioactivities of monotropein, such as anti-osteoporosis, anti-inflammation, anti-oxidation, anti-nociception, and hepatic or renal protection, are closely associated with its capability of downregulating the nuclear factor-κB signaling pathway, inhibiting the mitogen-activated protein kinase signaling pathway, attenuating the activation of nuclear factor E2-related factor 2/heme oxygenase-1 signaling pathway, and regulating the mammalian target of rapamycin/autophagy signaling pathway. However, the clinically therapeutic effects and the potential problems need to be addressed. This review highlights the current research progress on monotropein, which provides a reference for further investigation of monotropein.

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Background Pyrola decorata H. Andres, is exclusively distributed in China and a source of traditional Chinese herbal medicine Luxiancao for more than 2000 years. Here, we evaluated the antioxidant and cytoprotective effects of P. decorata and its five phenolic components (protocatechuic acid, gallic acid, hyperoside, 2''-O-galloylhyperin, and quercetin), and discussed their antioxidant chemistry. Methods A lyophilized aqueous extract of P. decorata (LAEP) was prepared and analyzed with high-performance liquid chromatography (HPLC). LAEP and its five phenolic components were comparatively investigated using five antioxidant assays, including ferric-reducing antioxidant power, cupric ion-reducing antioxidant capacity, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical (PTIO • )-scavenging, 1,1-diphenyl-2-picryl-hydrazl radical (DPPH • )-scavenging, and 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) radical (ABTS +• )-scavenging activities. The reaction products of the five phenolic components with 4-methoxy-2,2,6,6-tetramethylpiperidine-1-oxyl radical (4-methoxy-TEMPO • ) were determined with ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) analysis. LAEP and its five phenolic components were incubated with bone marrow-derived mesenchymal stem cells (bmMSCs) subjected to oxidative stress to demonstrate their cytoprotective effects with a flow cytometry assay. Results In the five antioxidant assays, LAEP and its five phenolic components dose-dependently increased the radical-scavenging (or reducing power) activities. However, the IC 50 values of hyperoside were consistently higher than those of 2''-O-galloylhyperin. UPLC-ESI-Q-TOF-MS/MS analysis results indicated that the five phenolics could yield dimer products in the presence of 4-methoxy-TEMPO • via the radical adduct formation (RAF) pathway. Flow cytometry assay results confirmed the cytoprotective activity of LAEP and its five phenolic components toward stressed bmMSCs. In particular, 2''-O-galloylhyperin could more effectively reduce the percentage of damaged bmMSCs than hyperoside. Conclusion LAEP and its five phenolic components may undergo redox-based pathways (such as electron transfer and H + transfer) and covalent-based pathway (i.e., RAF) to exhibit antioxidant activity. One consequence of RAF is the generation of phenolic-phenolic dimer. In both organic and aqueous media, 2''-O-galloylhyperin exhibited higher redox-based antioxidant levels (or cytoprotective levels) than those with hyperoside. The differences could be attributed to 2''-O-galloylation reaction.

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Background The aim of the study was to investigate knowledge and use of wild food plants in two mountain valleys separated by Mount Taibai--the highest peak of northern China and one of its biodiversity hotspots, each adjacent to species-rich temperate forest vegetation. Methods Seventy two free lists were collected among the inhabitants of two mountain valleys (36 in each). All the studied households are within walking distance of primary forest vegetation, however the valleys differed in access to urban centers: Houzhenzi is very isolated, and the Dali valley has easier access to the cities of central Shaanxi. Results Altogether, 185 wild food plant species and 17 fungi folk taxa were mentioned. The mean number of freelisted wild foods was very high in Houzhenzi (mean 25) and slightly lower in Dali (mean 18). An average respondent listed many species of wild vegetables, a few wild fruits and very few fungi. Age and male gender had a positive but very low effect on the number of taxa listed.Twelve taxa of wild vegetables (Allium spp., Amaranthus spp., Caryopteris divaricata, Helwingia japonica, Matteucia struthiopteris, Pteridium aquilinum, Toona sinensis, Cardamine macrophylla, Celastrus orbiculatus, Chenopodium album, Pimpinella sp., Staphylea bumalda &S. holocarpa), two species of edible fruits (Akebia trifoliata, Schisandra sphenanthera) and none of the mushrooms were freelisted by at least half of the respondents in one or two of the valleys. Conclusion The high number of wild vegetables listed is due to the high cultural position of this type of food in China compared to other parts of the world, as well as the high biodiversity of the village surroundings. A very high proportion of woodland species (42%, double the number of the ruderal species used) among the listed taxa is contrary to the general stereotype that wild vegetables in Asia are mainly ruderal species. The very low interest in wild mushroom collecting is noteworthy and is difficult to explain. It may arise from the easy access to the cultivated Auricularia and Lentinula mushrooms and very steep terrain, making foraging for fungi difficult.

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A liquid chromatography-mass spectrometry method was developed and validated for the quantitative determination of seven triterpenoids, 3-beta-O-alpha-L-arabinopyranosylsiaresinolic acid-28-O-beta-D-glucopyranosyl ester, ziyuglycoside I, pomolic acid, maslinic acid, colosic acid, oleanolic acid and ursolic acid in Pyrola decorata H. Andres. Chromatographic separation was achieved on a Hypersil C18 column using isocratic elution followed by a linear gradient elution of methanol and water as mobile phase. The analytes were ionized by atmospheric pressure chemical ionization source and determined on selected ion monitoring mode. All analytes showed good linearity (r2 > or = 0.9984) within the test ranges and the recovery rates were 94.5% - 103.3%. Satisfactory precision and reproducibility were obtained with relative standard deviation less than 5%. The method was simple, accurate and performed well in application to the determination of twenty commercial samples of P. decorata collected from different regions of China. It could be used for the quality control of both plant materials and preparations of P. decorata.

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