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There are three pathways for triacylglycerol (TAG) biosynthesis: De novo TAG biosynthesis, phosphatidylcholine-derived biosynthesis, and cytosolic TAG biosynthesis. Variability in fatty acid composition is mainly associated with phosphatidylcholine-derived TAG pathway. Mobilization of TAG-formed through cytosolic pathway into lipid droplets is yet unknown. There are multiple regulatory checkpoints starting from acetyl-CoA carboxylase to the lipid droplet biogenesis in TAG biosynthesis. Although a primary metabolism, only a few species synthesize oil in seeds for storage, and less than 10 species are commercially exploited. To meet out the growing demand for oil, diversifying into newer sources is the only choice left. The present review highlights the potential strategies targeting species like Azadirachta , Callophyllum , Madhuca , Moringa , Pongamia , Ricinus , and Simarouba , which are not being used for eating but are otherwise high yielding (ranging from 1.5 to 20 tons per hectare) with seeds having a high oil content (40-60%). Additionally, understanding the toxin biosynthesis in Ricinus and Simarouba would be useful in developing toxin-free oil plants. Realization of the importance of cell cultures as "oil factories" is not too far into the future and would soon be a commercially viable option for producing oils in vitro, round the clock.

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DNA barcoding helps to identify species, especially when identification is based on parts of organisms or life stages such as seeds, pollen, wood, roots or juveniles. However, the implementation of this approach strongly depends on the existence of complete reference libraries of DNA sequences. If such a library is incomplete, DNA-based identification will be inefficient. Here, we assess if DNA barcoding can already be implemented in species-rich tropical regions. We focus on the tree flora of São Paulo state, Brazil, which contains more than 2000 tree species. Using new DNA sequence data and carefully assembled GenBank accessions, we assembled 12,113 sequences from ten different regions. The ITS, rbcL, psbA-trnH, matK and trnL regions were better represented within the available sequences for São Paulo tree flora. Currently, only 58% of the São Paulo tree flora currently have at least one barcoding sequence available. However, these species represent on average 89% of the trees in São Paulo state forests. Therefore, conservation-oriented and ecological studies can already benefit from DNA barcoding to obtain more accurate species identifications. We present which taxa remain underrepresented for the São Paulo tree flora and discuss the implications of this result for other species-rich tropical regions.

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Quantitative GLC of triglycerides has been extended to natural fats containing both odd and even carbon number fatty acids. A 1.83-m glass column containing 3.0% JXR silicone on 100/120 mesh Gas-Chrom Q resolved triglycerides differing by only one carbon number. Peak resolution was significantly improved by hydrogenating each triglyceride sample prior to GLC analysis.The triglycerides of four fish oils (mullet, tuna, menhaden, and pilchard) and one seed fat (Acanthosyris spinescens) containing odd carbon number fatty acids were analyzed by this technique. The method was also useful for determining the triglyceride composition of the cyclopentene fatty acid oil fromHydnocarpus wightiana seeds.

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